用Clampfit计算动作电位阈值的方法.docx

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1、用Clampfit计算动作电位阈值的方法以Clampfit自带的样本数据为例，采用的数据文件为SynaPSOl.abf中的第一个Sweepo1 .打开CIamPfko2 .打开SynaPSoLabf文件。3 .点击计算功能的快捷键图标（下图中红色箭头所示）。富Clampfit-synaps01.abf-Analysis台FilewAnalyzeEventDetectionConfigureToolsWindowHelp里理匐国0点I圜固囹圃mlBl翼|国|画网硼ITVWH曳磨IH困凶JJI；命S)60-4 .出现下图的窗口5.在窗口中Expression的地方输入t2=diff(tl)6.点击

2、OK,可见下图，出现SIOPe曲线。*ClMnpIIt (syMp01 A*iytol口FIaCdt AraMS r vc CMtacam Crtft9x U *dw* 2品日寻Ool。当&阳miIBI4周/，P? -kt 心：IL，工限.1 QQ7.在Edit菜单中打开/Transfertraces,做下图（左侧）中的设定。TransferTraces念Clampfit-Results5-Results圜EdltViewAndyzeFormatEventDetectioncGResultswindowrGraphwindowpf,SCtlOll.IRegiontotransfer:FullU

3、ace：I(traceStarttimebaseatzerofpVariableStartTimeRegion一*j联IfflII备展牛/CAlignstarttimeswithfirstregionTime(ms)Trace#1Tracett2D6Keeporiginalstarttimespacing10-61.4206-614.20620.1-61.26641.54194TraceSelection302-61.2921-0.256958SelectIAS，gna，S40.3-61,26640.256958IAllvisibletraces50.4-61.24070.25699660.5

4、-61.3178-0.77095OKICancelIHelp70.6-60.032912.849580.7-57.642923.900190.8-55.818318.24638.点击OK后，会出现ReSUltS窗口,100.9-54.353414.6484可见右侧数据。111-52.991413.6205121.1-51.65513.36359.数据中第一个SlOPe点数据特别131.2-50.447212.0786141.3-49.162212.8495大，此为首端效应，耍将它去掉。151.4-48.031511.3076161.5-46.952110.793611PTrjirf4#1N名甘

5、宗火大Y171.6-45.821311.30761UJZk-11dCCfti9zxXX以tzVz，181.7-44.74210.7936选上Trace#2为，将其设定为Y+o191.8-43.739710.022611.点击CreatgraPh快捷图标（圆圈IJ201.9-42.737510.0226所示）。出现所做的图（下图）。212-41.78669.5086412.通过图中阈值的位置观察数据222.1-40.758610.2796232.2-39.78219.76563SIOPe的结果，发现阈值为-21.9984,242.3-38.88268.99466即22mV。252.4-38.03

6、458.48069262.5-37.0589.76563272.6-36.15858.99462282.7-35.23339.25164292.8-34.35968.73767302.9-33.43449.25164313-32.5358.99468323.1-31.66128.73765333.2-30.81318.48069343.3-30.06787.45269353.4-29.09139.76563363.5-28.29467.96672373.6-27.49797.96668383.7-26.62428.73767393.8-25.67339.50862403.9-24.79958.73769lClaspfit-Graph-Sheet14:2EBELtFiltEditViewAnalyzeEventDetectionConfigureToolsYindovHelpH呼lImg：;1凰巴陶IMT圆HIlfH曳MlHl鱼IBIfI帆|朗鸵国MajorTitleMinorTitIe100阈值位置LegendTrace#2S33el