Abstract101Pilotstudyoforalmicrobiomeandriskofpancreaticcancer.docx

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1、POSTERPRESENTA11ONS-PROFFEREDABSTRACTS,APRIL152013Abstract101:Pilotstudyoforalmicrobiomeandriskofpancreaticcancer.I-HsinLin;JingWu;StevenM.Cohen;CalvinChen;DarrenBryk;MollieMarr;MarcovalerioMelis;ElliotNewman;HLeonPachter;AlexanderV.Alekseyenko;RichardB.Hayes;JiyoungAhnAuthor&ArticleInformationCance

2、rRes(2013)73(8_Supplement):101.https:/doi.Org/10.1158/1538-7445.AM2013-101 Split-Screen ShareIconShare ToolsIconTooIs ArticleVersionsIconVersionsAbstractPreviousstudiesshowedthatperiodontitisandtoothlossareassociatedwithincreasedriskofpancreaticcancer,suggestingpossibleroleoforalbacteriainthepancrea

3、ticcanceretiology.Weevaluatedwhetheroralmicrobiomeisassociatedwithriskofpancreaticcancerandpancreatitis.Oralwashsampleswerecollectedfrom13pancreaticcancerpatients,3pancreatitispatientsand12healthycontrolsatNYULangoneMedicalCenter,NewYork.Ribosomal16SrRNAgenesfromoralwashDNAwereamplifiedbyuniversalpr

4、imers,barcoded,andsequencedwith454FLXtechnology.Thebacterial16SrRNAgenesequenceswerebinnedintooperationaltaxonomicunits(OTUs)with97%identifyandfurtherclassifiedbytaxonomy.NonparametricWilcoxonMannWhiteneyUtestandKuskal-Wallistestwereconductedtocomparetherelativeabundanceoftaxaacrosspancreaticcancer,

5、pancreatitisandcontrolgroups.Atotalof250,008sequencereads(average8,928readspersample)wereobserved.Firmicutes,Bacteroidetes,andActinobacteriaaremajorphyla,accountingfor96.5%oforalmicrobiota.Wefoundthatbacteroidesgenuswassignificantlymoreabundantinbothpancreaticcancerpatients(p=0.002)andpancreatitispa

6、tients(p=0.004)comparedwithcontrolgroup.Further,BLASTsearchresultsindicatethatthespeciesmostcloselyrepresentedbytheBacteroidesOTUswereoralpathogen.Inaddition,Corynebacterium(p=0.033)andAggregatibacter(p=0.019)werelessabundantinpancreaticcancerandpancretitisgroupscomparedwithcontrols.Datafromthispilo

7、tstudyoforalmicrobiomeandpancreaticcancersuggestthatthedifferentialabundanceinbacteriodatesandpossiblyCorynebacteriumandAggregatibacter5areassociatedwithriskofpancreaticcancer.Bacteroides,Gram-negativebacillusbacteria,playsanimportantroleinoralinflammation,includingperiodontitis.Furtherlargerstudies

8、arewarrantedtoconfirmthesepreliminaryfindings.CitationFormat:I-HsinLin,JingWu,StevenM.Cohen,CalvinChen,DarrenBryk5MollieMarr,MarcovalerioMelis,ElliotNewman,HLeonPachter,AlexanderV.Alekseyenko5RichardB.Hayes,JiyoungAhn.Pilotstudyoforalmicrobiomeandriskofpancreaticcancer,abstract.In:Proceedingsofthe104thAnnualMeetingoftheAmericanAssociationforCancerResearch;2013Apr6-10;Washington,DC.Philadelphia(PA):AACR;CancerRes2013;73(8SUPPI):AbStraCtnr101.doi:10.1158/1538-7445.AM2013-101