常用酶切位点表(含保护碱基).docx

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1、酶寡核昔酸序列切割率2hr20hrAcclGGTCGACCCGGTCGACCGCCGGTCGACCGG000000AfllllCacatgtgccacatgtggcccacatgtGGG0909009090AsclGGCGCGCCAggcgcgcctttggcgcgCCAA909090909090AvalCCCCGGGGCCCCCGGGGGTCCCCCGGGGGA509090909090BamHICGGATCCGCGGGATCCCGCGCGGATCCGCG109090259090BglllCagatctggaagatcttcggaagatctTCC0752509090BssHIIGGCGCGC

2、CAggcgcgcctttggcgcgCCAA00500090BstEIIGGGT(AT)ACCC010BstXIAactgcagaaccaatgcattggaaaacTgcagccaatgcattggaactgcagaaCcaatgcattggatgcat0252505090ClalCatcgatggatcgatcccatcgatggcCcatcgatggg009050009050EcoRIGgaattcccggaattccgccggaattcCGG909090909090HaelllGGGGccccAgcggccgctttgcggccGCAA909090909090HindlllCaagc

3、ttgccaagcttggcccaagcttGGG00100075KpnlGGGTACCCGGGGTACCCCCGGGGTACCCCG0909009090MlulGACGCGTCCGACGCGTCG025050NcolCccatgggCatgccatggcatg050075NdelCcatatggcccatatgggCgccatatgGCGGggtttcatatgaaacccggaattCcatatggaattccGggaattccatatgGaattccc0000757500009090NhelGgctagccCggctagccgctagctagcTAG0101002550寡核昔酸序列切割率

4、2hr20hrNotlTtgcggccgcaaAtttgcggccgctttaaaAtatgcggccgctataaaAtaagaatgcggCcgctaaactatAaggaaaaaagcggccgcAAAAGGAAAA010102525010109090NsilTgcatgcatgcaCcaatgcattggttctgcAGTT10909090PaclTtaattaaGttaattaacCcttaattaagg00002590PmelGtttaaacggtttaaaccGggtttaaacccAgctttgtttaaacggcgcgccgg000750255090PstlGctgcagcT

5、gcactgcagtgcaaactgcaGaaccaatgcattggAaaactgcagccaatGcattggaaCtgcagaaccaatgcattggaTGCAT0109090001090900PvulCcgatcggAtcgatcgatTcgcgatcgcgA010002510SaclCGAGCTCG1010SacllGccgcggcTccccgcgggga050090SallGtcgacgtcaaaaggccatagcggccgcGCgtcgacgtcttggccatagcggccgcggAcgcgtcgacgtcggccatagcggccgcgGAA0101005075欢迎共阅S

6、ealGagtactcaaaagtaciiii10752575SmalCCCGGGCCCCGGGGCCCCCGGGGGTCCCCCGGGGGA00109010105090SpelGactagtcGgactagtcccggactagtccGCtagactagtctag10100090905050SphlggcatgccCatgcatgcatgacatgcatgCATGT001002550StulAAGGCCIIGAAGGCCIICAAAAGGCCIlll909090909090XbalCtctagaggctctagagcTgctctagagcaCtagtctagactag09075750909090XholCCTCGAGGCCCTCGAGGGCCGCTCGAGCGG0101002575XmalCCCCGGGGCCCCCGGGGGCCCCCCGGGGGGTccccccgggggga02550900759090