First report of leaf spot on Angelica japonica caused by Septoria aegopodina in China.docx

Firstreportof1.eafspotonAnge1.icajaponicacausedbySeptoriaaegopodinainChina4 H.J.Xu.R.J.Zhou.1.EZhao.1.EFu.andN.HaoDepartmentofP1.antPatho1.ogy.Co1.1.egeofP1.ant5Protection.ShenyangAgricu1.tura1.University.Shenyang.1.iaoning.110866.China67 Ange1.icajaponicaA.Gray.aperennia1.herbintheUmbe1.1.a1.esfami1.y,iswide1.ydistributedinChina8 asamedicina1.herbandvegetab1.e.InAUgUS1.2015,IeafSPOtsymptomswereobservedon1.eavesof9 A.japonicainther(x1.uctionI1.e1.dsofDengchangvi1.1.age,Xinbin,1.iaoning,China.Thedisease10 main1.yaffectedthe1.eavesandstems,withsymptomsoccurringonapproximate1.y25to50%ofthe11 p1.antsreducingyie1.dbyabout30%.Initia1.1.y,symptomsdeve1.opedonbothsidesof1.eavesas12 scatteredpinpoint1.ightbrownspots.As(he1.esionsexpanded,theydeve1.opedintopa1.ebrown,13 e1.1.iptica1.orirregu1.ar1.yshaped1.esionsthateventua1.1.ybecamedryandgrayishorwhiteinco1.or14 bearingpycnidiasurroundedbybrownvio1.etnecroticmargins.Numerous1.esionsbecamecracked15 atthecentersandcoa1.escedtogether.CondiaIbnnedinIhepycnidiaweret1.1.ifo11nto16 fi1.iform-cy1.indrica1.,Stniightorcurved,gradua1.1.yattenuatedtoanarrow1.yroundedtopointedapex,17 andInOrCor1.essattenuatedtowardsthenarrow1.ytruncatebase.Theywere(0)1.-3-scptate.hya1.ine.18 withsevera1.oi1.-drop1.eisandminutegranu1.armateria1.ineachce1.1.,21.4to42.7×1.4to2.5rn19 (average26.5×2.1m),Thepathogenwasiso1.atedfromsymptomatictissues,andcu1.turedon20 PDAat25for2to3wk.Theco1.onywasrestrictedandSOmCwhate1.evated,producingdense.21 whitetograyaeria1.myce1.iumthat1.aterbecamedarkerwithanaveragegrowthrateof0.35nn11day.22 After20d.s1.imy,sa1.mon-co1.oredconidia1.masseswereinterspersedwithintheco1.onics.Sequences23 ofsix1.oci,trans1.atione1.ongationfactorI-a1.pha(tcfI).beta-tubu1.in(TUB),RNApo1.ymeraseI1.24 second(R1,B2).1.argestsubunit1.argesubunitribosoma1.(1.SU),interna1.transcribedspacer(ITS),25 andca1.modu1.in(CA1.),wereamp1.ifiedwithprimersEFI-728Fef-2,T1-Sandy-R,26 fRPB2-5FfRPB2-41.4R.1.SUIFd,1.R5,ITS5ITS4,andCA1.-235FCA1.2Rd.respective1.y,and27 sequenced(Quaedv1.iegeta1.2013;Verk1.eyeta1.2013).Thephy1.ogenetictreewasproducedusing28 MECA4.0,andneighbor-joiningana1.yseswereerfonnedusingthecombineddatasetsofthesix29 regions,formedasing1.ec1.adewithmaximumsupport(100%)withsequencesofSeptoria3()aego)odinaSacc.'I"heresu1.tingsequences,tef1.(550bp).TUB(480bp).RPB2(367bp).1.SU(88631bp),ITS(507bp),andCA1.(422bp)weredepositedinGenBankasKU921437toKU92I453and32 KT985904.respective1.y.Thus,thefunguswasidentifiedasS.aegopdinaonthebasisof33 morpho1.ogica1.andcu1.tura1.characteristics(Verk1.eyeta1.2013)andmu1.ti-1.ocusphy1.ogeneticana1.ysis34 (Verideyeta1.2013).Toconfinnthepathogenicity,fivedetached1.eavesofA.japonicawere35 inocu1.atedwithaConidia1.suspension(IO6conidiam1.1).Contro1.1.eavesweretreatedwithsteri1.e36 disti1.1.edwateron1.y.The1.eavesweremaintainedinap1.asticcontainerinthegreenhouse(re1.ative37 humidityabout95%)at25innatura1.day1.ightandobservedperi<xiicaHy.Symptomson1.eaves,38 identica1.Iothoseobservedinthefie1.ds,werevisib1.e8toIOdaysafterinocu1.ation.No1.esions39 deve1.opedonthecontro1.1.eaves.Thesamefunguswasre-iso1.atedfromthesymptomatictissue.40 identifiedbymorpho1.ogy,comp1.etingKoch'spostu1.ates.oourknow1.edge,thisisthefirstforma1.41 reportof1.eafspotonA.japonicacausedbyS.aeg<>p<>dinain1.iaoning,Chinaanditmaybeusefu1.42 intheestab1.ishmentofabaseforscientificCon1.rO1.ofthedisease.Theinfo11nationdescribedhere43 mayhe1.pgrowersmanagethedisease.4445 Keferences:46 W.Quacdv1.icgcta1.StudMyco1.75:307,2013.47 GJ.M.Vcrk1.cycta1.StudMyco1.75:213,2013.