Identification and molecular characterization of Powdery Mildew of Clematis manshurica Caused by Erysiphe aquilegiae in China.docx

Identificationandmo1.ecu1.archaracterizationofPowderyMi1.dewofC1.ematismanshuricaCausedbyErysiphea<ui1.egiaeinChinaMorpho1.ogica1.andmo1.ecu1.aridentificationOfPowdCryMi1.dewofC1.ematismanshuricaCausedbyErysipheaquHegiaeinChinaRuJun3m>u.HaiJi;NXu.YimXHuiOv.NingHw.JunEanF.DearTC(1.ofPImKP(h<1.f¼'.CdkjeofPUniProcti(XkShcn)nAgrhzUkUrn1.Un1.VCTXhy.Sbenyw5.1.ijiming.H0$i66.ChinaC1.eniatumt/Hihitncai,upr.isaperennia1.herbintheKanuiKruIacvaetan1.y.whichhasbeenusedasuVu1.uab1.c(raditiow1.Chinesemodici,Between2012and2013.aPOWdCrymi1.dewcausingsignifkan1.damagetnp1.antsofGmanshuricaintheMa1.icimi1.HerbGardeninShenyangAgricu1.tura1.Universi1.y.Uaoningprovince.China.ha»<ruxred.'1.efungii11feciedmain1.ythe1.eaves,theMa1.ksandpetio1.escana1.sobeinfectedinsevereinfections.Symptomsinitia1.1.ya>edredascircu1.artovu1.ar.thu.WhMec。IOnie«on1.x>thsidesof1.ea%*eax1.youngstems,especia1.1.yontheadaxia1.surface.Andeventua1.1.yinfectionsusua1.1.ycausedye1.1.owingofthe1.eave%am!Tnii1.fnrrndtinnundpremature<kt<)1.iati().Thefun!uxfmmym>Unu1.ctissueswas>kn1.if>edasEmipheucui1.eae(Er5spaks.AscomyaHa>b%edonInorPhOiQgiCa1.CharaCtCriJiCsPhy!<>gcncicana1.ysisbasedonrDNASshowedthu(theSNi2<MIK>strainfa1.1.inIbeNamewe11-Mpottedchfe.whichisc1.。W1.yrehedIoErysiffJie蚓I1.Negiae.pathogenici1.ytoorigina1.hostwasCOnAfTnodthroughinocu1.ationc.pcrincncs.To<miknow1.edge、thisKIhcf1.rxtrcp>r1.ofpnvderymikkwcmC.ManShMriCQOIga1.byE.tqui1.eiatinChina.Introduction/Ittt1.iItSNSPJngDDoC×.<<1.rwrWcmIherc<X8&WrhU(X11QCM11atmnshufta(J).Zatschnttfur31.rIorschtngBJCornaiO1.Cmtoa*S6n,2006.61110)1299-1303.CwfuIiughHficaPUPrisPerCrWia1.herbintheRanUncubCCaCIumi1.y.andiswide1.ydistributedinthex>rthcas(ofChina.Thedriedrootandrhizonsofthep1.antsnamed“WeiIingxiaEintheCInDCSCPhanMCoPOCEispopu1.ar1.yusedaxanUntiinf1.amnuitory,ant(unr.ana1.gesicagentinUaditiuiu1.Cbinewnedicinefor1.horiadsofyea¼inAsi4StiiSPeIJ20OdInA1.IRUMof2012.NPOwdCTymi1.dewnC.QIanShUriaIwmfirMobservedinMedicina1.IIcrbGardenofShenyangAgriCUhUni1.Universityin1.iaoningprovince.Chinxduringinvestigationofp1.antdivca%conC.ntanhuric<j.IncidenceofPowderMi1.dewofC.mttuhuriixwasnve(gated.andIhgMXIp1.ainswevco1.1.ectedTheincidenceofthediseasewasratedintermsofpercentageofinfbctodIEvCSj11xg1()()1.avcsobservedwithfiverep1.icates.The1.eve1.otincidencewasXigniGcantIydifferentmtwoyears.In2012.theincidencewasIowcrJusiunder5%.However,in20)3.thediseaseincidencesharp1.yircascd.with42%(o100%(nvcngcof78%)inthesamefickhInthispaper,(1.ieaim½aIdemifyDiecausa1.agentofx>wdeymi1.dew<>bsenedonC1.etnatiinMHi1.11trainChiiwandseiupabaseforSCiCnUnCcontro1.ofthedisease.Theribosoma1.DNAinterna)CninscribedspacerI!TS>regionhasbeenusefu1.fordiscriminatingfngi(thespeciesfcvd(i81.1.ftifc<Vr£ft-j妥Zh1：).andhasbeenUWdex(evne1.ytorUujI1.k1.MIfKrM>nui>!yk>genetana1.ysisof×>uderyImkkvI1.KI4"Hr2001.k05tJamesH.CUnn1.nton2003.200.Wrag.HiXX).k<kogd)Materia1.sandmethodsFunga1.materia1.sandmkroscop)Thesan>1.esofC1.emuiisrruuuhitricant'ecedw1.hxwderymi1.dewwerec>btauedfrntheMcdiciiw1.HcrtiGardenofShenyangAgriehUni1.University.1.iaoningprovic.China.Themorpho1.ogyofthep;I1.bQgCn、wereexaminedfrcUmii1.eriahuing1.ightmicroscopyaspreviousdescnbed心1e1.a2001).!)NAextractionandamp1.1.f1.cath>nOrri)NAITSfttt1.2t1.Ghk.KTA.Sn.GS.Cumi1.mJ.H.TuImuIkuS.,H«uc.I.B0b,1.NcPCS>n,Y.<11JRmgmA.Y.f2X1.1.INdgv<softwomM*fmp.CAif1.Mtr*¾atvrzm»pnew；fidO枚KTWMinfnt<j<njcindrfcrcMpc<t(Ikr*r<UX1>v<hk>1.MgUM1.III*6M-6Q1*ttN3上Wmxkkr“ku出"tfd*w厢MwW,r*MIbffCxmmIIXXgof，R，E1.1.Twrt>hiuUeoMi1.ikw即MoUiCiXo<hmc<kII上Comrf1.tonJH.1.*eAC.PasooeIG.UnpctodQosonu1.DNArtecnaitrarcrt>01.epaw山Ms9州KEr>peaoutj8scnsuioJ.Funna1.Oversf/.2004.16:110.Meh:M1.rMcnti1.kut<<<*v11iMAUCOr*ku><E>>iMEe1.,u<da*i*nf%<P*1.1.2mXf).32.421-42«t-ItttIC”Hi>xT.<*M11ti11tmJHZSiti.U.1.inicMSMU-StmhbMf.N.Gr½>1.uruc.B.$J»,YtTAa11"b<u.S.1.2(KOE<dniy*u1.>gf>Mh<vfr<UvjjvUxuIttt116UKOVgGM,W-KiWIV89gcG(he11rOMATSwquenewo(o？ne*>wymkSffspecKesdoroU311mD½cuUrKiCra3300«AWN(JeVa1.uggiNorrveMOfPpurc<.M1.V<7fQJrdjiiykv；rK«NCEr1.KiJiwfV，1.pu,dnyHii1.dewtiMiin(heUK*IX上KiSS1.CookRIA.SaonzGS.1.akSerf1.11whonHk>POWe<ymMIUngi*tt11$上NxtooidCSOqg3<mwsityCtrDNAsre3IracQtwdspacerCX1.rac1.edIrwnCOnddar1.cieetc<hecacfSeVOraIr-Ktt!IO:fiu*vCBM<w*c"mIntrAkp<tk4<t>h1.1.1.1.IafeffrMnmm1.i11cdFrUXmer1.1.1.x11<h1.,nurhh三nahFU呻>1DNAMimp1.cswerePrCPdrCdhyco1.1.ectingCkiQmheci;IITc1.Nuy;iF1.irataax1.SuuimuTakamaisu/HDdyoungodcrynk1.wmye1.iaUSingSwri1.Cbuhcj.Jankovics.2001.Thenuc1.earrDNAregion%panmngtheITSk5.8S»ndS2rRNgenewasUmPIHia1.byPO1.ymeraWchainreaction(PCR).PrimersITS!<F»TS4We1.Eusedfortheam1.ifkaion.whichwasperformedinatow!reactionvo1.umeof501.inc1.udingthefo1.1.o