细胞悬浮体系的建立0.docx
蝴电悬浮体系的建立及成分测定Estab1.ishmentofCe1.1.suspensioncu1.turesofDictainiuisdasycarpusfortheproductionofbioactiveComP(HIndS(Dictaminine)Obacunone(黄柏Jraxine1.1.one(样),1.im<,nin(柠株苦素)Dictamnine(白鲜)目的：I.建立Censuspensionprotoco1.：2,确定影响产量的因素3.确定最优配方内Mt.P1.antmateria1.andestab1.ishmentofce1.1.suspensionCa1.iUS的培养：黄期已建立的体系(引用发表文章)ce1.1.suspensioncu1.ture(用原配方2.0gFW=50m1.250m1.=J过速=反夏SUbCU1.IUre2.PGRsconcentrationandcombination激素选择：2.4-D,NAA.IBA：0.1.0.5.1.0.2.0.5.0mg1.激素组合：2.0mg1.2.4-D+KTorBAP：0.1.0.5.1.0.2.0mg1.3 .c1.trecondition(1) Mediastrength1.1/2、1/3,1法、1.0MS(2) Inocu1.umdensity：2.5.5,10.15,20.25gFW(3) CarbonSOUrCC(ConCCn1.raIiOn”I,2,3,4,5,6,7%(4) Photoperiod：Dark.16-1.ight,24-1.igh1.(5) Initia1.pH:4.0.5.().5.5.6.0.6.5,7.0.8.0.9.0.10.0(6)KII2P()4concentration(7) Mg2+Concentration：370mg1.(8) NH4+NO3:总量(1:1)20-100：比率:60:0=10:604 .(>11>wthcurve(kinetics)1 -8weeks5 .Deterninationofce1.1.biomassI1.irough0.45umstain1.essste1.1.sieve<sigma).FWandDW(60度)6 .ExtractionandHP1.Cana1.ysisDiscussion：1 .激素种类对生长和含量的影响；241)对生长的影啕，对成分的影响；2 .光照对生长日I成分含量的影3 .初始PH对生长量和成分含量的影响；4 .Mg2+对生长量的影响及其机制(8气若奉甲0途径)5 .NII4+浓度对生长和含的影响及其机制(4K基笨甲“途役)Estab1.ishmentofCe1.1.suspensioncu1.turesofDictamnusdasycarpusfortheproductionof1.>ioac1.ivecompounds(Dictamiiiiiw)Estab1.ishmentofCe1.1.suspensioncu1.turesofDictamnusdasycarpusandbiosynthesisofactivecompounds(Dictaminine)AbstractKeywords:Introduction写作思路：药用植物介绍(是个重妥的药用植物，作为传统中药；功效：活性)主要成分是植物本身含，低(Zusua1.1.yvery1.ow)f1.Recent1.y9重要成分Ahasattractedinterestedduetoitsstrong的功效，活性，治疗的疾病，A在植物上veryminorproductionThereforea1.ternativeexp1.orationof*»isimportant,的前人研究进展hasbeenreportedin"*本文研究内容Materia1.sandmethodsP1.antmateria1.andestab1.ishmentofce1.1.suspensionInf1.uenceofPGRsonbiomassandtheProdUCtionofbioactivecompoundsOptimizationofcu1.tureconditions(1) Mediastrength：1/2、1/3、1/4、I.OMS(2) Inocu1.umdensity：2.55.10.15.20.25g1.FW(3) Carbonsource(concentration)1.23,4,5.6,7%(4) Photoperiod:Darkt16-1.ight,24-1.ight(5) Initia1.pH:4.0,5.0,556.0.6.5.7.0.8.0.9.0.10.0(6)Mg2+Concentrationt370mg1.(7)NH4÷Concentrations1.650mg1.Growthcurve(kinetics)Determinationofce1.1.biomassQuantitativeana1.ysisofbioactivecompounds引用自己文章ExtractspreparationandquantitativeHP1.Cana1.ysesofactivecompoundswereerfo11>ed,accordingtotheproceduresdescribedprevious1.y引自己ft*文章)Statistica1.(Data)ana1.ysisA1.1.experimenta1.datawerestatistica1.1.yana1.yzedbyonewayana1.ysisofvariance(ANOVA)usingtheprotectedIeaSt-SignifiCanI-difference(1.SD)test(P<0.05).andwerepresentedbyaverage±standarderror(SE).Kesu1.tsanddiscussion生长素Conccntni1.i«»n<mp1.FreehWeight®Dr,Weightg>Contentsofacthecompounds!mgDw)CYIIStat1.INOtMiCUn<>ihFraxindhmc1.in>nin1.>kUmniH*24D0.00.110.2±0.05IO.2±O.O3102±0.0310.2£0.0310.2±0.03102±0.03Pakye1.1.ow.Itugik0，1.O2.0DKydMw,cixnptc1.5.0NAA0.00.10、1.02.05.0IBA0.00.10，1.02.05.0分裂索（固定2.4-D为2.0m1.）ConCentriItionImg/11Frc*ihWeight<g)>JVeht(»>ContentsofactiveCOinPOUndMm田£DW)Ce1.1.statusOImcuim>iwKmuwUnvIJmaninIMctamnineKIN0.00.11O.2±O.O310.2±0.03IO.2±O.O3102±0.0310.2±0.03oj±o.Pa1.cyd!ow.fragi1.eOJ1.02.0Daik>,t!1.uw.CDmpOC1.5.0BAP0.()0.10.51.02.05.0Mediastrength序号MSStrengthFrexhWeight®Dr，Weight(g>Contcntxofactivec<mpondxmg,DW)Ce1.1.statusObavunoneFraxine1.1.oneIJmoninDidamnineIIM20.2±0.03IO.2±O,O3IO.2±O.O310.2±0.03IO.2±O.O310.2±0.03Pa1.cye1.1.ow,fragi1.e31/241.0Inocu1.umdensity序号Inocu1.umdensityW1.)FrWhWeight(g)DryWeight1.j>>ContentsofacthtrEnpoUndSimagDW>Oi1.!UUMOhucunoncFnixinc1.1.one1.im<ninDictnmnineI2.5025.00IO.2O.O310.2±0.0310.2i0.0310.210.03IOJ±0.03IO.2±O.O3Pa1.c),c1.1.ow.fragi1.e310.0415.0520.0625.0Carbonconcentration»号CarbonConcentmti<>n(%)FFhWeigh!(f>)DryWeht(>C.,(>nten1.soactiec<mpoundsmgDW>Ce1.1.%ta1.uxObavunoneFraxine1.1.oneIJmoninOidamnineI02I10.2±0.0310.2±0.0310.2±0.03IO.2±O.O31.<2±O.O310.2±0.03Pa1.cye1.1.ow.Fragi1.e3243546576Photo>cri<x1.用号PhiMoperiOdFrthDryWdght)Con
